論文 - 詳細
| RRC ID | 28172 |
|---|---|
| 著者 | Murayama Y, Uhlmann F. |
| タイトル | Biochemical reconstitution of topological DNA binding by the cohesin ring. |
| ジャーナル | Nature |
| Abstract |
Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin. |
| 巻・号 | 505(7483) |
| ページ | 367-71 |
| 公開日 | 2014-1-16 |
| DOI | 10.1038/nature12867 |
| PII | nature12867 |
| PMID | 24291789 |
| PMC | PMC3907785 |
| MeSH | Adenosine Triphosphatases / metabolism Cell Cycle Proteins / chemistry* Cell Cycle Proteins / metabolism* Chromatids / genetics Chromatids / metabolism Chromosomal Proteins, Non-Histone / chemistry* Chromosomal Proteins, Non-Histone / metabolism* DNA / chemistry* DNA / metabolism* DNA-Binding Proteins / metabolism Nucleic Acid Conformation* Protein Binding Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism |
| IF | 42.779 |
| 引用数 | 145 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | cDNA library |