RRC ID 28172
著者 Murayama Y, Uhlmann F.
タイトル Biochemical reconstitution of topological DNA binding by the cohesin ring.
ジャーナル Nature
Abstract Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin.
巻・号 505(7483)
ページ 367-71
公開日 2014-1-16
DOI 10.1038/nature12867
PII nature12867
PMID 24291789
PMC PMC3907785
MeSH Adenosine Triphosphatases / metabolism Cell Cycle Proteins / chemistry* Cell Cycle Proteins / metabolism* Chromatids / genetics Chromatids / metabolism Chromosomal Proteins, Non-Histone / chemistry* Chromosomal Proteins, Non-Histone / metabolism* DNA / chemistry* DNA / metabolism* DNA-Binding Proteins / metabolism Nucleic Acid Conformation* Protein Binding Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism
IF 42.779
引用数 145
WOS 分野 CELL BIOLOGY
リソース情報
酵母 cDNA library