RRC ID 28437
Author Nikaido T, Shimizu A, Ishida N, Sabe H, Teshigawara K, Maeda M, Uchiyama T, Yodoi J, Honjo T.
Title Molecular cloning of cDNA encoding human interleukin-2 receptor.
Journal Nature
Abstract The human interleukin-2 (IL-2) receptor was purified by affinity chromatography using the anti-Tac monoclonal antibody, and its N-terminal amino acid sequence was determined. Complementary DNA clones were isolated and sequenced to reveal the primary structure of the IL-2 receptor precursor, which has 272 amino acid residues. The receptor is separated into two domains by a putative 19-residue transmembrane region. Two mRNAs (1.4 and 3.5 kilobases) hybridizing to the cDNA clone were found in human T cells bearing the IL-2 receptor. The cDNA directed synthesis of the IL-2 receptor in COS cells.
Volume 311(5987)
Pages 631-5
Published 1984-10-18
DOI 10.1038/311631a0
PMID 6090949
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular* DNA / isolation & purification DNA / metabolism* DNA Restriction Enzymes Genes* Genetic Vectors Humans Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger / genetics Receptors, Antigen, T-Cell / genetics* Receptors, Immunologic / genetics* Receptors, Immunologic / isolation & purification Receptors, Interleukin-2 Transcription, Genetic
IF 42.779
Times Cited 601
DNA material pKCR. Tac-2A (RDB01171)