RRC ID 28447
著者 Hatta K, Nose A, Nagafuchi A, Takeichi M.
タイトル Cloning and expression of cDNA encoding a neural calcium-dependent cell adhesion molecule: its identity in the cadherin gene family.
ジャーナル J Cell Biol
Abstract The neural cadherin (N-cadherin) is a Ca2+-dependent cell-cell adhesion molecule detected in neural tissues as well as in non-neural tissues. We report here the nucleotide sequence of the chicken N-cadherin cDNA and the deduced amino acid sequence. The sequence data suggest that N-cadherin has one transmembrane domain which divides the molecule into an extracellular and a cytoplasmic domain; the extracellular domain contains internal repeats of characteristic sequences. When the N-cadherin cDNA connected with virus promoters was transfected into L cells which have no endogenous N-cadherin, the transformants acquired the N-cadherin-mediated aggregating property, indicating that the cloned cDNA contained all information necessary for the cell-cell binding action of this molecule. We then compared the primary structure of N-cadherin with that of other molecules defined as cadherin subclasses. The results showed that these molecules contain common amino acid sequences throughout their entire length, which confirms our hypothesis that cadherins make a gene family.
巻・号 106(3)
ページ 873-81
公開日 1988-3-1
DOI 10.1083/jcb.106.3.873
PMID 2831236
PMC PMC2115086
MeSH Amino Acid Sequence Animals Antigens, Surface / genetics* Base Sequence Brain Chemistry Cell Adhesion Cell Adhesion Molecules Cell Aggregation Cell Communication Chickens Cloning, Molecular DNA / genetics* DNA Restriction Enzymes Genes* L Cells Molecular Sequence Data Neurons / analysis* Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transfection
IF 8.811
引用数 380
WOS 分野 CELL BIOLOGY
オルトメトリクス指標
オルトメトリクス指標項目
最多言及媒体 Patent(IFI CLAIMS)
各媒体での言及数の合計 25
過去6か月間でのオルトメトリクス指標の変動値 0.0
リソース情報
遺伝子材料 Chicken N-cadherin full length cDNA (RDB01187).