論文 - 詳細
| RRC ID | 28454 |
|---|---|
| 著者 | Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J. |
| タイトル | Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Transcription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The POU factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal of pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, and A. G. Smith, Nat. Genet. 24:372-376, 2000). Here we have developed a complementation assay based on the ability of Oct-3/4 transgenes to rescue self-renewal in conditionally null ES cells and used this to define which domains of Oct-3/4 are required to sustain the undifferentiated stem cell phenotype. Surprisingly, we found that molecules lacking either the N-terminal or C-terminal transactivation domain, though not both, can effectively replace full-length Oct-3/4. Furthermore, a fusion of the heterologous transactivation domain of Oct-2 to the Oct-3/4 POU domain can also sustain self-renewal. Thus, the unique function of Oct-3/4 in ES cell propagation resides in combination of the specific POU domain with a generic proline-rich transactivation domain. Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation. |
| 巻・号 | 22(5) |
| ページ | 1526-36 |
| 公開日 | 2002-3-1 |
| DOI | 10.1128/MCB.22.5.1526-1536.2002 |
| PMID | 11839818 |
| PMC | PMC134688 |
| MeSH | Animals Cells, Cultured DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Embryo, Mammalian / cytology* Genetic Complementation Test Humans Mice Mutation Octamer Transcription Factor-3 Protein Binding Protein Structure, Tertiary Sequence Deletion Stem Cells / physiology* Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation* |
| IF | 3.611 |
| 引用数 | 221 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | ptk-luc (RDB07031) pFGF4tk-luc (RDB07032) p6W5'tk-luc (RDB07033) phUTF1tk-luc (RDB07034) pPyCAG-BstXI-IP (RDB07099) pPyCAG-cHA-IP (RDB07100) pBRPyCAG-cHA-IP (RDB07101) pPyCAG-Oct3wt-IRESpuropA (RDB09609) ptk-luc Fgf4enh3' (RDB09711) p052e/p-luc (RDB09725) pRex1p-luc (RDB09723) pmPGKp-luc (RDB09790). |