RRC ID 2859
Author Matsuda M, Shinomiya A, Kinoshita M, Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, Hamaguchi S, Sakaizumi M, Nagahama Y.
Title DMY gene induces male development in genetically female (XX) medaka fish.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of the teleost fish medaka (Oryzias latipes). DMY has been shown to be required for the normal development of male individuals. In this study, we show that a 117-kb genomic DNA fragment that carries DMY is able to induce testis differentiation and subsequent male development in XX (genetically female) medaka. In addition, overexpression of DMY cDNA under the control of the CMV promoter also caused XX sex reversal. These results demonstrate that DMY is sufficient for male development in medaka and suggest that the functional difference between the X and Y chromosomes in medaka is a single gene. Our data indicate that DMY is an additional sex-determining gene in vertebrates.
Volume 104(10)
Pages 3865-70
Published 2007-3-6
DOI 10.1073/pnas.0611707104
PII 0611707104
PMID 17360444
PMC PMC1820675
MeSH Animals Animals, Genetically Modified Base Sequence DNA, Complementary / metabolism Female Genes, sry* Male Models, Genetic Molecular Sequence Data Oryzias / genetics* Phenotype Promoter Regions, Genetic Sex Determination Processes* Sex Differentiation X Chromosome*
IF 9.504
Times Cited 116
Medaka d-rR HdrR-II1 (IB178) Qurt (MT828) Hd-rR.Y^HNI (MT191) HNI-II (IB176)