RRC ID 28642
Author Matsumoto Y, Marusawa H, Kinoshita K, Endo Y, Kou T, Morisawa T, Azuma T, Okazaki IM, Honjo T, Chiba T.
Title Helicobacter pylori infection triggers aberrant expression of activation-induced cytidine deaminase in gastric epithelium.
Journal Nat. Med.
Abstract Infection with Helicobacter pylori (H. pylori) is a risk factor for the development of gastric cancer. Here we show that infection of gastric epithelial cells with 'cag' pathogenicity island (cagPAI)-positive H. pylori induced aberrant expression of activation-induced cytidine deaminase (AID), a member of the cytidine-deaminase family that acts as a DNA- and RNA-editing enzyme, via the IkappaB kinase-dependent nuclear factor-kappaB activation pathway. H. pylori-mediated upregulation of AID resulted in the accumulation of nucleotide alterations in the TP53 tumor suppressor gene in gastric cells in vitro. Our findings provide evidence that aberrant AID expression caused by H. pylori infection might be a mechanism of mutation accumulation in the gastric mucosa during H. pylori-associated gastric carcinogenesis.
Volume 13(4)
Pages 470-6
Published 2007-4
DOI 10.1038/nm1566
PII nm1566
PMID 17401375
MeSH Antigens, Bacterial / metabolism Bacterial Proteins / metabolism Cytidine Deaminase / metabolism* DNA Primers Gastric Mucosa / metabolism* Gene Expression Regulation, Neoplastic* Genes, p53 / genetics* Helicobacter Infections / genetics* Helicobacter Infections / metabolism Humans Immunohistochemistry Models, Biological Mutagenesis / genetics NF-kappa B / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / physiology Stomach Neoplasms / genetics* Stomach Neoplasms / microbiology
IF 32.621
Times Cited 274
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY MEDICINE, RESEARCH & EXPERIMENTAL CELL BIOLOGY
Resource
DNA material pCMV5a-AID-c-Myc-His (RDB08057)