Reference - Detail
|Author||Shibuya T, Watanabe K, Yamashita H, Shimizu K, Miyashita H, Abe M, Moriya T, Ohta H, Sonoda H, Shimosegawa T, Tabayashi K, Sato Y.|
|Title||Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin.|
|Journal||Arterioscler. Thromb. Vasc. Biol.|
OBJECTIVE:We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile.
METHODS AND RESULTS:By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSIONS:These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.
|MeSH||Angiogenesis Inhibitors / analysis Angiogenesis Inhibitors / genetics* Angiogenesis Inhibitors / pharmacology Animals Cells, Cultured Endothelial Cells / chemistry Humans Immunohistochemistry Mice Neovascularization, Physiologic / drug effects Organ Culture Techniques RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction|
|WOS Category||HEMATOLOGY PERIPHERAL VASCULAR DISEASE|
|DNA material||p3XFLAG-CMV-14-hVASH2 (RDB08519)|