RRC ID 28655
Author Shibuya T, Watanabe K, Yamashita H, Shimizu K, Miyashita H, Abe M, Moriya T, Ohta H, Sonoda H, Shimosegawa T, Tabayashi K, Sato Y.
Title Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin.
Journal Arterioscler Thromb Vasc Biol
Abstract OBJECTIVE:We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile.
METHODS AND RESULTS:By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice.
CONCLUSIONS:These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.
Volume 26(5)
Pages 1051-7
Published 2006-5-1
DOI 10.1161/01.ATV.0000216747.66660.26
PII 01.ATV.0000216747.66660.26
PMID 16528006
MeSH Angiogenesis Inhibitors / analysis Angiogenesis Inhibitors / genetics* Angiogenesis Inhibitors / pharmacology Animals Cells, Cultured Endothelial Cells / chemistry Humans Immunohistochemistry Mice Neovascularization, Physiologic / drug effects Organ Culture Techniques RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction
IF 6.604
Times Cited 66
DNA material p3XFLAG-CMV-14-hVASH2 (RDB08519)