論文 - 詳細
| RRC ID | 28658 |
|---|---|
| 著者 | Hirao K, Hata Y, Yao I, Deguchi M, Kawabe H, Mizoguchi A, Takai Y. |
| タイトル | Three isoforms of synaptic scaffolding molecule and their characterization. Multimerization between the isoforms and their interaction with N-methyl-D-aspartate receptors and SAP90/PSD-95-associated protein. |
| ジャーナル | J Biol Chem |
| Abstract |
The synaptic scaffolding molecule (S-SCAM) has been identified as a protein interacting with SAP90/PSD-95-associated protein (SAPAP) (also called guanylate kinase-associated protein/hDLG-associated protein). S-SCAM has six PDZ (we have numbered them PDZ-0 to -5), two WW, and one guanylate kinase (GK) domains and interacts with N-methyl-D-aspartate (NMDA) receptor via PDZ-5 and SAPAP via the GK domain. We have identified here shorter isoforms of S-SCAM that start at the 164th or 224th methionine, and we renamed the original one, S-SCAMalpha, the middle one, S-SCAMbeta, and the shortest one, S-SCAM-gamma. S-SCAMbeta and -gamma have five PDZ (PDZ-1 to -5), two WW, and one GK domains. S-SCAMalpha interacted with S-SCAMbeta and -gamma through the region containing PDZ-4 and -5. The region containing both of PDZ-4 and -5 is sufficient for the clustering of NMDA receptors and forms a dimer in gel filtration, suggesting that S-SCAM forms multimers via the interaction between the C-terminal PDZ domains and assembles NMDA receptors into clusters. S-SCAMbeta and -gamma also interacted with SAPAP, suggesting that the N-terminal region of the GK domain is not necessary for the interaction. Finally, we have identified the interaction of the PDZ domains of S-SCAM with the GK domain of PSD-95/SAP90. S-SCAM, PSD-95/SAP90, and SAPAP are colocalized at least in some part in brain. Therefore, S-SCAM, PSD-95/SAP90, and SAPAP may form a complex in vivo. |
| 巻・号 | 275(4) |
| ページ | 2966-72 |
| 公開日 | 2000-1-28 |
| DOI | 10.1074/jbc.275.4.2966 |
| PII | S0021-9258(18)31062-7 |
| PMID | 10644767 |
| MeSH | Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Base Sequence Biopolymers CHO Cells COS Cells Carrier Proteins / chemistry Carrier Proteins / metabolism* Cerebellum / metabolism Chromatography, Gel Cricetinae DNA Primers Guanylate Kinases Microscopy, Fluorescence Molecular Sequence Data Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / metabolism* Protein Binding Protein Isoforms / chemistry Protein Isoforms / metabolism* Rats Receptors, N-Methyl-D-Aspartate / metabolism* Retina / metabolism SAP90-PSD95 Associated Proteins Sequence Homology, Amino Acid |
| IF | 4.238 |
| 引用数 | 55 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | S-SCAM (p3205-54) (RDB03435) |