RRC ID 28663
Author Katsuyama T, Sugawara T, Tatsumi M, Oshima Y, Gehring WJ, Aigaki T, Kurata S.
Title Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification.
Journal Proc Natl Acad Sci U S A
Abstract How organ identity is determined is a fundamental question in developmental biology. In Drosophila, field-specific selector genes, such as eyeless (ey) for eyes and vestigial (vg) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye (wge), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge-induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-(Antennapedia) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent (eya), a control gene for eye development downstream of ey, in eye discs. In contrast to vg, however, overexpression of wge inhibited EY-mediated expression of eya. Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc. These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes.
Volume 102(44)
Pages 15918-23
Published 2005-11-1
DOI 10.1073/pnas.0507945102
PII 0507945102
PMID 16247005
PMC PMC1276095
MeSH Animals Base Sequence Chromatin / metabolism Chromosomal Proteins, Non-Histone / genetics* DNA-Binding Proteins / genetics Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / genetics* Eye / growth & development Forkhead Transcription Factors / genetics* Gene Expression Regulation, Developmental* Molecular Sequence Data Morphogenesis / genetics Organ Specificity / genetics* Wings, Animal / growth & development
IF 9.412
Times Cited 13
DNA material CG31151 full/pUAST-F (RDB07965) deltaBAH/pUAST-F (RDB07966)
Drosophila DGRC#206414