論文 - 詳細
| RRC ID | 28663 |
|---|---|
| 著者 | Katsuyama T, Sugawara T, Tatsumi M, Oshima Y, Gehring WJ, Aigaki T, Kurata S. |
| タイトル | Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
How organ identity is determined is a fundamental question in developmental biology. In Drosophila, field-specific selector genes, such as eyeless (ey) for eyes and vestigial (vg) for wings, participate in the determination of imaginal disc-specific identity. We performed gain-of-function screening and identified a gene named winged eye (wge), which encodes a bromo-adjacent homology domain protein that localizes at specific sites on chromosomes in a bromo-adjacent homology domain-dependent manner. Overexpression of wge-induced ectopic wings with antero-posterior and dorso-ventral axes in the eye field in a region-specific Hox gene-(Antennapedia) independent manner. Overexpression of wge was sufficient for ectopic expression of vg in eye discs. A context-dependent requirement of wge was demonstrated for vg expression in wing discs and for expression of eyes absent (eya), a control gene for eye development downstream of ey, in eye discs. In contrast to vg, however, overexpression of wge inhibited EY-mediated expression of eya. Consistent with colocalization on polytene chromosomes of WGE and Posterior sex combs (PSC), a Polycomb group gene product, we demonstrated an antagonistic genetic interaction between wge and Psc. These findings suggest that wge functions in the determination of disc-specific identity, downstream of Hox genes. |
| 巻・号 | 102(44) |
| ページ | 15918-23 |
| 公開日 | 2005-11-1 |
| DOI | 10.1073/pnas.0507945102 |
| PII | 0507945102 |
| PMID | 16247005 |
| PMC | PMC1276095 |
| MeSH | Animals Base Sequence Chromatin / metabolism Chromosomal Proteins, Non-Histone / genetics* DNA-Binding Proteins / genetics Drosophila / genetics* Drosophila / growth & development Drosophila Proteins / genetics* Eye / growth & development Forkhead Transcription Factors / genetics* Gene Expression Regulation, Developmental* Molecular Sequence Data Morphogenesis / genetics Organ Specificity / genetics* Wings, Animal / growth & development |
| IF | 9.412 |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | CG31151 full/pUAST-F (RDB07965) deltaBAH/pUAST-F (RDB07966) |
| ショウジョウバエ | DGRC#206414 |