Reference - Detail
| RRC ID | 28686 |
|---|---|
| Author | Saito R, Nakayama A. |
| Title | Differences in malate dehydrogenases from the obligately piezophilic deep-sea bacterium Moritella sp. strain 2D2 and the psychrophilic bacterium Moritella sp. strain 5710. |
| Journal | FEMS Microbiol Lett |
| Abstract |
The gene encoding malate dehydrogenase (MDH) of the obligately piezophilic deep-sea bacterium Moritella sp. strain 2D2 was cloned and sequenced. There were two positions [close to the active site (Ala-180) and in the subunit interaction site (His-229)] with 2D2-specific substitutions. The MDH genes of strain 2D2 and a psychrophilic bacterium Moritella sp. strain 5710 exhibiting the highest sequence similarity were overexpressed in Escherichia coli. The 2D2 MDH was more heat-stable than the 5710 MDH. The apparent Km value at 62.1 MPa for NADH of the 2D2 MDH was higher than that of the 5710 MDH. The 2D2 MDH in which a His-Gln substitution was introduced at position 229 decreased the thermal stability and Km value at 62.1 MPa. The 5710 MDH that was substituted Gln-229 with His increased the thermal stability and Km value at 62.1 MPa. These results indicate that the His residue at position 229 of the 2D2 MDH may play a role in the thermal stability and the MDH function at high pressure. |
| Volume | 233(1) |
| Pages | 165-72 |
| Published | 2004-4-1 |
| DOI | 10.1016/j.femsle.2004.02.004 |
| PII | S0378109704001193 |
| PMID | 15043884 |
| MeSH | Amino Acid Sequence Amino Acid Substitution Cloning, Molecular Cold Temperature DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Enzyme Stability Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Genes, Bacterial Genes, rRNA Hydrostatic Pressure Kinetics Malate Dehydrogenase / chemistry Malate Dehydrogenase / genetics Malate Dehydrogenase / isolation & purification Malate Dehydrogenase / metabolism* Molecular Sequence Data Moritella / enzymology* Moritella / genetics Moritella / physiology Mutagenesis, Site-Directed Mutation, Missense Phylogeny RNA, Ribosomal, 16S / genetics Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Seawater / microbiology* Sequence Alignment Sequence Analysis, DNA |
| IF | 1.987 |
| Times Cited | 10 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pET41b FactorXa 2D2 MDH (RDB08301) pET41b FactorXa H229Q 2D2 MDH (RDB08302) pET41b FactorXa 5710 MDH (RDB08303) pET41b FactorXa Q229H 5710 MDH (RDB08304) |