| RRC ID |
28821
|
| Author |
Shimizu K, Sano T, Kubota R, Kobayashi N, Tahara M, Obama T, Sugimoto N, Nishimura T, Ikarashi Y.
|
| Title |
Effects of the amino acid constituents of microcystin variants on cytotoxicity to primary cultured rat hepatocytes.
|
| Journal |
Toxins (Basel)
|
| Abstract |
Microcystins, which are cyclic heptapeptides produced by some cyanobacterial species from algal blooms, strongly inhibit serine/threonine protein phosphatase and are known as hepatotoxins. Microcystins have many structural variations, yet insufficient information is available on the differences in the cytotoxic potentials among the structural variants. In this study, the cytotoxicities of 16 microcystin variants at concentrations of 0.03-10 μg/mL to primary cultured rat hepatocytes were determined by measuring cellular ATP content, and subsequently determined by their 50% inhibitory concentration (IC50). Differences in the amino acid constituents were associated with differences in cytotoxic potential. [D-Asp3, Z-Dhb7] microcystin-LR exhibited the strongest cytotoxicity at IC50 of 0.053 μg/mL among the microcystin variants tested. Furthermore, [d-Asp3, Z-Dhb7] microcystin-HtyR was also highly cytotoxic. These results suggest that both D-Asp and Z-Dhb residues are important in determining the cytotoxic potential of microcystin variants.
|
| Volume |
6(1)
|
| Pages |
168-79
|
| Published |
2013-12-30
|
| DOI |
10.3390/toxins6010168
|
| PII |
toxins6010168
|
| PMID |
24380975
|
| PMC |
PMC3920255
|
| MeSH |
Amino Acids / chemistry*
Animals
Cells, Cultured
Hepatocytes / drug effects*
Hepatocytes / metabolism
Inhibitory Concentration 50
Marine Toxins
Microcystins / chemistry
Microcystins / toxicity*
Phosphoprotein Phosphatases / metabolism
Rats
Rats, Sprague-Dawley
|
| IF |
3.531
|
| Times Cited |
23
|
|
WOS Category
|
TOXICOLOGY
|
| Resource |
| Algae |
NIES-90
NIES-610
NIES-928
NIES-595 |
