Reference - Detail
| RRC ID | 2882 |
|---|---|
| Author | Taniguchi Y, Takeda S, Furutani-Seiki M, Kamei Y, Todo T, Sasado T, Deguchi T, Kondoh H, Mudde J, Yamazoe M, Hidaka M, Mitani H, Toyoda A, Sakaki Y, Plasterk RH, Cuppen E. |
| Title | Generation of medaka gene knockout models by target-selected mutagenesis. |
| Journal | Genome Biol |
| Abstract |
We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes. |
| Volume | 7(12) |
| Pages | R116 |
| Published | 2006-1-1 |
| DOI | 10.1186/gb-2006-7-12-r116 |
| PII | gb-2006-7-12-r116 |
| PMID | 17156454 |
| PMC | PMC1794429 |
| MeSH | Adenosine Triphosphatases / genetics Animals Animals, Genetically Modified* Base Sequence DNA Helicases / genetics DNA Primers Ethylnitrosourea / administration & dosage Female Genes, p53 Male Models, Genetic* Mutagenesis, Site-Directed Oryzias / genetics* Point Mutation RecQ Helicases Ubiquitin-Protein Ligases / genetics |
| IF | 10.806 |
| Times Cited | 99 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| Resource | |
| Medaka | MT893 MT894 WS338 TILLING_MUTANT |