| RRC ID |
28836
|
| 著者 |
Kuramoto T, Inoue S, Neoda Y, Yamasaki K, Hashimoto R, Mashimo T, Oda S, Serikawa T.
|
| タイトル |
Genetic and phenotypic characterization of a Japanese wild-derived DOB/Oda rat strain.
|
| ジャーナル |
Mamm Genome
|
| Abstract |
Wild-derived rat strains can provide novel genome resources that are not available in standard laboratory strains. Genetic backgrounds of wild-derived strains can facilitate effective genetic linkage analyses and often modulate the expression of mutant phenotypes. Here we describe the development and characterization of a new inbred rat strain, DOB/Oda, from wild rats (Rattus norvegicus) captured in Shitara, Aichi, Japan. Phenotype analysis of 109 parameters revealed that the DOB/Oda rats had small body weight, preference for darkness, and high locomotor activity compared with the rat strains in the National BioResource Project for the Rat (NBRP-Rat) database. Genome analysis with 357 SSLP markers identified DOB/Oda-specific alleles in 70 markers. The percentage of SSLP markers that showed polymorphism between the DOB/Oda strain and any of 132 laboratory strains from NBRP-Rat varied from 89 to 95 %. The polymorphic rate (average of the values of the percentage) for the DOB/Oda strain was 91.6 %, much higher than the rates for available wild-derived strains such as the Brown Norway rat. A phylogenic tree constructed with DOB/Oda and all the strains in NBRP-Rat showed that the DOB/Oda strain localized within the wild rat groups, apparently separate from the laboratory strains. Together, these findings indicated that the DOB/Oda rat has a unique genome that is not available in the laboratory strains. Therefore, the new DOB/Oda strain will provide an important genome resource that will be useful for designing genetic experiments and for the discovery of genes that modulate mutant phenotypes.
|
| 巻・号 |
24(7-8)
|
| ページ |
303-8
|
| 公開日 |
2013-8-1
|
| DOI |
10.1007/s00335-013-9465-z
|
| PMID |
23896813
|
| MeSH |
Animals
Body Weight / genetics
Breeding
Female
Genome / genetics
Genotype
Japan
Male
Phenotype
Polymorphism, Genetic
Rats
Rats, Inbred Strains / genetics*
|
| IF |
2.287
|
| 引用数 |
1
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| リソース情報 |
| ラット |
DOB/Oda(strainID=582) |
