Reference - Detail
| RRC ID | 28841 |
|---|---|
| Author | Miki TS, Rüegger S, Gaidatzis D, Stadler MB, Großhans H. |
| Title | Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover. |
| Journal | Nucleic Acids Res |
| Abstract |
Although XRN2 proteins are highly conserved eukaryotic 5'→3' exonucleases, little is known about their function in animals. Here, we characterize Caenorhabditis elegans XRN2, which we find to be a broadly and constitutively expressed nuclear protein. An xrn-2 null mutation or loss of XRN2 catalytic activity causes a molting defect and early larval arrest. However, by generating a conditionally mutant xrn-2ts strain de novo through an approach that may be also applicable to other genes of interest, we reveal further functions in fertility, during embryogenesis and during additional larval stages. Consistent with the known role of XRN2 in controlling microRNA (miRNA) levels, we can demonstrate that loss of XRN2 activity stabilizes some rapidly decaying miRNAs. Surprisingly, however, other miRNAs continue to decay rapidly in xrn-2ts animals. Thus, XRN2 has unanticipated miRNA specificity in vivo, and its diverse developmental functions may relate to distinct substrates. Finally, our global analysis of miRNA stability during larval stage 1 reveals that miRNA passenger strands (miR*s) are substantially less stable than guide strands (miRs), supporting the notion that the former are mostly byproducts of biogenesis rather than a less abundant functional species. |
| Volume | 42(6) |
| Pages | 4056-67 |
| Published | 2014-4-1 |
| DOI | 10.1093/nar/gkt1418 |
| PII | gkt1418 |
| PMID | 24445807 |
| PMC | PMC3973323 |
| MeSH | Alleles Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Exoribonucleases / genetics Exoribonucleases / metabolism Exoribonucleases / physiology* MicroRNAs / metabolism* Molting / genetics Mutagenesis, Site-Directed Mutation RNA Stability Substrate Specificity Temperature |
| IF | 11.502 |
| Times Cited | 17 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3473 |