RRC ID 28933
Author Morrison A, Harwood A.
Title A simple method of generating axenic derivatives of Dictyostelium strains.
Journal Exp Cell Res
Abstract We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer.
Volume 199(2)
Pages 383-6
Published 1992-4-1
DOI 10.1016/0014-4827(92)90449-i
PMID 1544378
MeSH Animals Dictyostelium / growth & development* Germ-Free Life* Phenotype Plasmids
IF 3.329
Times Cited 12
WOS Category CELL BIOLOGY ONCOLOGY
Resource
Cellular slime molds S00470