Reference - Detail
RRC ID | 28935 |
---|---|
Author | Hadwiger JA, Firtel RA. |
Title | Analysis of G alpha 4, a G-protein subunit required for multicellular development in Dictyostelium. |
Journal | Genes Dev |
Abstract |
The Dictyostelium G alpha 4 gene encodes a G-protein alpha subunit that is primarily expressed during the multicellular stages of development. g alpha 4 null mutants, created by gene disruption, show aberrant morphological differentiation, reduced levels of prespore gene expression, and a loss of the ability to produce spores. These developmental phenotypes can be rescued by complementation with the wild-type gene. Cells that overexpress the G alpha 4 gene (G alpha 4HC) also show reduced spore production but display an aberrant morphological phenotype distinct from that of g alpha 4 cells. The g alpha 4 phenotype can be partially rescued by the presence of wild-type or G alpha 4HC cells in chimeric organisms, suggesting that G alpha 4-expressing cells produce an intercellular signal that is essential for multicellular development. |
Volume | 6(1) |
Pages | 38-49 |
Published | 1992-1-1 |
DOI | 10.1101/gad.6.1.38 |
PMID | 1730409 |
MeSH | Animals Base Sequence Cell Differentiation / genetics* DNA Mutational Analysis Dictyostelium / genetics* Dictyostelium / growth & development GTP-Binding Proteins / genetics* Gene Expression / genetics Genes, Fungal / genetics* Molecular Sequence Data Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction / genetics Spores, Fungal / genetics beta-Galactosidase / genetics beta-Galactosidase / metabolism |
IF | 9.527 |
Times Cited | 130 |
WOS Category | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
Resource | |
Cellular slime molds | S00057 S00182 |