Reference - Detail
| RRC ID | 28936 |
|---|---|
| Author | Witke W, Schleicher M, Noegel AA. |
| Title | Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins. |
| Journal | Cell |
| Abstract |
We generated by gene disruption Dictyostelium cells that lacked both the F-actin cross-linking proteins, alpha-actinin and gelation factor. Several major cell functions, such as growth, chemotaxis, phagocytosis, and pinocytosis, were apparently unaltered. However, in all double mutants, development was greatly impaired. After formation of aggregates, cells were very rarely able to form fruiting bodies. This ability was rescued when mutant and wild-type strains were mixed in a ratio of 70 to 30. The developmental program in the mutant was not arrested, since the expression pattern of early and late genes remained unchanged. Development of the mutant was rendered normal when a functional alpha-actinin gene was introduced and expressed, showing the morphogenetic defect to be due to the absence of the two F-actin cross-linking proteins. These findings suggest the existence of a functional network allowing mutual complementation of certain actin-binding proteins. |
| Volume | 68(1) |
| Pages | 53-62 |
| Published | 1992-1-10 |
| DOI | 10.1016/0092-8674(92)90205-q |
| PII | 0092-8674(92)90205-Q |
| PMID | 1732064 |
| MeSH | Actin Cytoskeleton / physiology* Actin Cytoskeleton / ultrastructure Actinin / genetics Actins / genetics* Actins / physiology Animals Carrier Proteins / genetics Cell Movement Chemotaxis Cytoskeleton / physiology Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / physiology* Genetic Vectors Microfilament Proteins / genetics Models, Biological Mutagenesis Phagocytosis Pinocytosis |
| IF | 38.637 |
| Times Cited | 134 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S00403 S00416 S90303 |