| RRC ID |
28944
|
| 著者 |
Barclay SL, Henderson EJ.
|
| タイトル |
Altered cyclic-AMP receptor activity and morphogenesis in a chemosensory mutant of Dictyostelium discoideum.
|
| ジャーナル |
Differentiation
|
| Abstract |
The functional properties of the cell-surface cyclic-AMP receptor that controls chemotaxis were found to be altered in an aggregation mutant of Dictyostelium discoideum. The mutant aggregated without stream formation and had a tenfold increased cell-density requirement for the initiation of aggregation. After aggregation, mounds formed multiple tips and subsequently subdivided to give multiple fruits that were small and abnormally proportioned. Cyclic-AMP-induced light-scattering changes in cell suspensions indicated that the mutant had a diminished response to external cyclic-AMP signals. Associated with these altered functional responses was a physical change in the cyclic-AMP sensory system. Cyclic-AMP-binding studies showed that the parent had two classes of cyclic-AMP binding sites, i.e., Kd = 32 and 110 nM. In contrast, the mutant had two- to threefold or more high-affinity sites (Kd = 25 nM) and altered low-affinity sites (Kd less than 3 microM). These results indicate that both affinity classes of binding site are independently mutable. This observation suggests that the two affinity classes can be interconverted by mutation, or the mutation alters a single molecular species and its equilibrium between binding sites with different affinities for cyclic AMP, as postulated in receptor cycling models.
|
| 巻・号 |
33(2)
|
| ページ |
111-20
|
| 公開日 |
1986-1-1
|
| DOI |
10.1111/j.1432-0436.1986.tb00416.x
|
| PII |
S0301-4681(11)60836-0
|
| PMID |
3032720
|
| MeSH |
Cyclic AMP / pharmacology
Dictyostelium / cytology
Dictyostelium / genetics
Dictyostelium / growth & development*
Kinetics
Morphogenesis
Mutation*
Receptors, Cyclic AMP / metabolism*
|
| IF |
2.392
|
| 引用数 |
7
|
|
WOS 分野
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S00341 |
