RRC ID 28968
Author Harwood AJ, Plyte SE, Woodgett J, Strutt H, Kay RR.
Title Glycogen synthase kinase 3 regulates cell fate in Dictyostelium.
Journal Cell
Abstract Extracellular cyclic AMP (cAMP) induces the formation of prespore cells in Dictyostelium but inhibits stalk cell formation. We have cloned gskA, which encodes the Dictyostelium homolog of glycogen synthase kinase 3 (GSK-3), and discovered that it is required for both cAMP effects. Disruption of gskA creates a mutant that aggregates but forms few spores and an abnormally high number of stalk cells. These stalk cells probably arise from an expanded prestalk B (pstB) cell population, which normally produces the basal disc of the fruiting body. In cultured mutant cells, cAMP neither inhibits pstB cell differentiation nor induces efficient prespore cell differentiation. We propose that cAMP acts through a common pathway that requires GSK-3 and determines the proportion of prespore and pstB cells.
Volume 80(1)
Pages 139-48
Published 1995-1-13
DOI 10.1016/0092-8674(95)90458-1
PII 0092-8674(95)90458-1
PMID 7813009
MeSH Amino Acid Sequence Animals Base Sequence Calcium-Calmodulin-Dependent Protein Kinases / chemistry Calcium-Calmodulin-Dependent Protein Kinases / genetics Calcium-Calmodulin-Dependent Protein Kinases / metabolism* Cell Differentiation Cyclic AMP / pharmacology Dictyostelium / cytology* Dictyostelium / enzymology* Dictyostelium / genetics Dictyostelium / physiology Extracellular Matrix Proteins / genetics Fungal Proteins / genetics Genes, Fungal Glycogen Synthase Kinase 3 Glycogen Synthase Kinases Molecular Sequence Data Mutation Phenotype Protozoan Proteins* Spores, Fungal / cytology
IF 38.637
Times Cited 176
Cellular slime molds S00140