RRC ID 28975
著者 Louis JM, Ginsburg GT, Kimmel AR.
タイトル The cAMP receptor CAR4 regulates axial patterning and cellular differentiation during late development of Dictyostelium.
ジャーナル Genes Dev
Abstract Pseudoplasmodia of developing Dictyostelium are organized with anteroposterior polarity. We have isolated CAR4, the gene for a new cell-surface, G protein-linked cAMP receptor. CAR4 mRNA is initially expressed during tip elongation and continues to accumulate into culmination. CAR4 is maximally expressed in pseudoplasmodia anteriors which are centers for extracellular cAMP signaling and for organization of cellular patterning. Although car4 null cells progress unperturbed through early development, they exhibit major patterning aberrations as the anteroposterior axis becomes established. Prestalk gene expression is significantly reduced in car4 nulls, whereas prespore-specific markers are overexpressed and detected in zones normally restricted to prestalk cells. Patterning defects are similarly apparent in terminally differentiated fruiting bodies. Our results show that cAMP signaling is required for pattern formation and cellular differentiation during late Dictyostelium development.
巻・号 8(17)
ページ 2086-96
公開日 1994-9-1
DOI 10.1101/gad.8.17.2086
PMID 7958880
MeSH Amino Acid Sequence Animals Cell Differentiation / genetics Cell Differentiation / physiology DNA, Complementary / genetics Dictyostelium / genetics Dictyostelium / growth & development* GTP-Binding Proteins / genetics GTP-Binding Proteins / physiology* Gene Deletion Gene Expression Regulation, Developmental Genes, Fungal Genes, Protozoan Genetic Markers Molecular Sequence Data Protozoan Proteins* Receptors, Cyclic AMP / genetics Receptors, Cyclic AMP / physiology* Recombination, Genetic Signal Transduction / genetics Signal Transduction / physiology
IF 9.527
引用数 72
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 S00240 S90404