RRC ID 28990
著者 Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH.
タイトル Dictyostelium RasG is required for normal motility and cytokinesis, but not growth.
ジャーナル J Cell Biol
Abstract RasG is the most abundant Ras protein in growing Dictyostelium cells and the closest relative of mammalian Ras proteins. We have generated null mutants in which expression of RasG is completely abolished. Unexpectedly, RasG- cells are able to grow at nearly wild-type rates. However, they exhibit defective cell movement and a wide range of defects in the control of the actin cytoskeleton, including a loss of cell polarity, absence of normal lamellipodia, formation of unusual small, punctate polymerized actin structures, and a large number of abnormally long filopodia. Despite their lack of polarity and abnormal cytoskeleton, mutant cells perform normal chemotaxis. However, rasG- cells are unable to perform normal cytokinesis, becoming multinucleate when grown in suspension culture. Taken together, these data suggest a principal role for RasG in coordination of cell movement and control of the cytoskeleton.
巻・号 138(3)
ページ 605-14
公開日 1997-8-11
DOI 10.1083/jcb.138.3.605
PMID 9245789
PMC PMC2141629
MeSH Actins / analysis Animals Cell Adhesion Cell Division* Cell Polarity Chemotaxis Cytoskeleton / chemistry Cytoskeleton / physiology Cytoskeleton / ultrastructure Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / growth & development Dictyostelium / physiology* Movement Mutation Myosins / physiology Pseudopodia / ultrastructure Transfection ras Proteins / genetics ras Proteins / physiology*
IF 8.811
引用数 108
WOS 分野 CELL BIOLOGY
リソース情報
細胞性粘菌 S00251