RRC ID 28997
Author Nagasaki A, Sutoh K, Adachi H, Sutoh K.
Title A novel Dictyostelium discoideum gene required for cAMP-dependent cell aggregation.
Journal Biochem. Biophys. Res. Commun.
Abstract Using a method of random insertional mutagenesis called REMI (restriction enzyme-mediated integration), we isolated two mutant strains of Dictyostelium discoideum with a defect in cAMP-dependent cell aggregation. On bacterial lawns, both of the cells formed large and smooth plaques. When starved in a non-nutrient medium, they became elongated and extended pseudopods very frequently like starved wild type cells. However, they never formed streams toward an aggregation center. Genomic DNA fragments flanking the sites of insertion of the REMI tag were rescued from the mutant cells. The fragments contained one common open reading frame encoding a protein of 1148 amino acid residues. The protein's sequence is homologous to those of two hypothetical proteins of S. cerevisiae and S. pombe.
Volume 244(2)
Pages 505-13
Published 1998-3-17
DOI 10.1006/bbrc.1998.8230
PII S0006-291X(98)98230-4
PMID 9514897
MeSH Amino Acid Sequence Animals Chemotaxis / drug effects Chemotaxis / genetics Cloning, Molecular Cyclic AMP / pharmacology* Dictyostelium / cytology Dictyostelium / drug effects* Dictyostelium / genetics* Fungal Proteins / genetics Gene Expression Gene Targeting Genes, Fungal* Genes, Protozoan* Molecular Sequence Data Mutation Phenotype Protozoan Proteins / genetics Saccharomyces cerevisiae / genetics Schizosaccharomyces / genetics Sequence Homology, Amino Acid
IF 2.559
Times Cited 15
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Cellular slime molds S00051 S00302 S00309 S00552