RRC ID 29002
著者 Yasukawa H, Mohanty S, Firtel RA.
タイトル Identification and analysis of a gene that is essential for morphogenesis and prespore cell differentiation in Dictyostelium.
ジャーナル Development
Abstract We have identified a gene (PslA) that is expressed throughout Dictyostelium development and encodes a novel protein that is required for proper aggregation and subsequent cell-type differentiation and morphogenesis. pslA null (pslA-) cells produce large aggregation streams under conditions in which wild-type cells form discrete aggregates. Tips form along the stream, elongate to produce a finger, and eventually form a terminal structure that lacks a true sorus (spore head). More than half of the cells remain as a mass at the base of the developing fingers. The primary defect in the pslA- strain is the inability to induce prespore cell differentiation. Analyses of gene expression show a complete lack of prespore-specific gene expression and no mature spores are produced. In chimeras with wild-type cells, pslA- cells form the prestalk domain and normal, properly proportioned fruiting bodies can be produced. This indicates that pslA- cells are able to interact with wild-type cells and regulate patterning, even though pslA- cells are unable to express prespore cell-type-specific genes, do not participate in prespore cell differentiation and do not produce pslA- spores in the chimeras. While pslA- cells produce mature, vacuolated stalk cells during multicellular development, pslA- cells are unable to do so in vitro in response to exogenous DIF (a morphogen required for prestalk and stalk cell differentiation). These results indicate that pslA- cells exhibit a defect in the prestalk/stalk cell pathways under these experimental conditions. Our results suggest that PslA's primary function is to regulate prespore cell determination very early in the prespore pathway via a cell-autonomous mechanism, possibly at the time of the initial prestalk/prespore cell-fate decision. Indirect immunofluorescence of myc-tagged PslA localizes the protein to the nucleus, suggesting that PslA may function to control the prespore pathway at the level of transcription.
巻・号 125(14)
ページ 2565-76
公開日 1998-7-1
DOI 10.1242/dev.125.14.2565
PMID 9636072
MeSH Amino Acid Sequence Animals Cell Aggregation / genetics Cell Differentiation / genetics* Chimera / genetics Cloning, Molecular Dictyostelium / growth & development* Fungal Proteins / chemistry* Gene Expression Regulation, Developmental / genetics Hexanones / pharmacology Histocytochemistry Microscopy Molecular Sequence Data Morphogenesis / physiology* Mutagenesis / genetics Nuclear Proteins / physiology Promoter Regions, Genetic / genetics Protozoan Proteins* RNA, Messenger / metabolism Sequence Analysis, DNA Transcription Factors / chemistry*
IF 5.611
引用数 7
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 S00327