RRC ID 29004
Author Anjard C, Chang WT, Gross J, Nellen W.
Title Production and activity of spore differentiation factors (SDFs) in Dictyostelium.
Journal Development
Abstract SDF-1 and SDF-2 are peptides that promote terminal spore differentiation under submerged conditions. The present study shows that they accumulate differentially and are released during the development of wild-type cells and can promote spore formation in cells disaggregated from wild-type culminants. SDF-1 accumulates during the slug stage and is released in a single burst at the onset of culmination while SDF-2 accumulates during early culmination and is released in a single burst from mid-culminants. The effects of SDF-1 and SDF-2 on stalk cell formation in cell monolayers were investigated. SDF-1 by itself induces stalk cell formation in some strains and also synergizes with the stalk-cell-inducing factor, DIF-1. cAMP has an inhibitory effect on stalk cell formation when either DIF-1 or SDF-1 are present on their own but is almost not inhibitory when both are present. SDF-2 alone does not induce stalk cell formation and appears to inhibit the response to DIF-1. At the same time, it increases the extent of vacuolization of the stalk cells that are produced. We propose that the release of SDF-1 and then of SDF-2 may mark irreversible steps in the developmental programme associated, respectively, with culmination and spore maturation.
Volume 125(20)
Pages 4067-75
Published 1998-10-1
PMID 9735367
MeSH Animals Caenorhabditis elegans Proteins* Carrier Proteins / pharmacology Cell Aggregation Cell Differentiation Cell Line Cyclic AMP / pharmacology Dictyostelium / cytology Dictyostelium / metabolism Dictyostelium / physiology* Dose-Response Relationship, Drug Helminth Proteins / pharmacology Hexanones Hydrocarbons, Chlorinated Life Cycle Stages Proteins* Protozoan Proteins / isolation & purification Protozoan Proteins / metabolism* Protozoan Proteins / pharmacology Spores / cytology Spores / growth & development Spores / metabolism Time Factors
IF 5.611
Times Cited 44
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Cellular slime molds S00334