| RRC ID |
29021
|
| 著者 |
Wilkins A, Khosla M, Fraser DJ, Spiegelman GB, Fisher PR, Weeks G, Insall RH.
|
| タイトル |
Dictyostelium RasD is required for normal phototaxis, but not differentiation.
|
| ジャーナル |
Genes Dev
|
| Abstract |
RasD, a Dictyostelium homolog of mammalian Ras, is maximally expressed during the multicellular stage of development. Normal Dictyostelium aggregates are phototactic and thermotactic, moving towards sources of light and heat with great sensitivity. We show that disruption of the gene for rasD causes a near-total loss of phototaxis and thermotaxis in mutant aggregates, without obvious effects on undirected movement. Previous experiments had suggested important roles for RasD in development and cell-type determination. Surprisingly, rasD(-) cells show no obvious changes in these processes. These cells represent a novel class of phototaxis mutant, and indicate a role for a Ras pathway in the connections between stimuli and coordinated cell movement.
|
| 巻・号 |
14(11)
|
| ページ |
1407-13
|
| 公開日 |
2000-6-1
|
| PMID |
10837033
|
| PMC |
PMC316659
|
| MeSH |
Animals
Blotting, Northern
Blotting, Southern
Blotting, Western
Cell Differentiation
Cell Division
Dictyostelium / cytology
Dictyostelium / genetics*
Light
Movement
Mutagenesis
Photosynthesis*
Signal Transduction
Temperature
Transfection
ras Proteins / genetics*
ras Proteins / physiology*
|
| IF |
9.527
|
| 引用数 |
40
|
|
WOS 分野
|
GENETICS & HEREDITY
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S00249 |
