論文 - 詳細
| RRC ID | 29033 |
|---|---|
| 著者 | Müller-Taubenberger A, Lupas AN, Li H, Ecke M, Simmeth E, Gerisch G. |
| タイトル | Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis. |
| ジャーナル | EMBO J |
| Abstract |
Calreticulin and calnexin are Ca2+-binding proteins with chaperone activity in the endoplasmic reticulum. These proteins have been eliminated by gene replacement in Dictyostelium, the only microorganism known to harbor both proteins; family members in Dictyostelium are located at the base of phylogenetic trees. A dramatic decline in the rate of phagocytosis was observed in double mutants lacking calreticulin and calnexin, whereas only mild changes occurred in single mutants. Dictyostelium cells are professional phagocytes, capable of internalizing particles by a sequence of activities: adhesion of the particle to the cell surface, actin-dependent outgrowth of a phagocytic cup, and separation of the phagosome from the plasma membrane. In the double-null mutants, particles still adhered to the cell surface, but the outgrowth of phagocytic cups was compromised. Green fluorescent protein-tagged calreticulin and calnexin, expressed in wild-type cells, revealed a direct link of the endoplasmic reticulum to the phagocytic cup enclosing a particle, such that the Ca2+ storage capacity of calreticulin and calnexin might directly modulate activities of the actin system during particle uptake. |
| 巻・号 | 20(23) |
| ページ | 6772-82 |
| 公開日 | 2001-12-3 |
| DOI | 10.1093/emboj/20.23.6772 |
| PMID | 11726513 |
| PMC | PMC125758 |
| MeSH | Animals Animals, Genetically Modified Blotting, Southern Calcium / metabolism Calcium-Binding Proteins / metabolism Calcium-Binding Proteins / physiology* Calnexin Calreticulin Cell Adhesion Chemotaxis DNA, Complementary / metabolism Dictyostelium Endoplasmic Reticulum / metabolism* Genetic Vectors Green Fluorescent Proteins Luminescent Proteins / metabolism Mice Mice, Inbred BALB C Microscopy, Fluorescence Models, Genetic Mutation Phagocytosis Phylogeny Protein Binding Protein Structure, Tertiary Recombinant Fusion Proteins / metabolism Ribonucleoproteins / metabolism Ribonucleoproteins / physiology* Time Factors Transformation, Genetic |
| IF | 9.889 |
| 引用数 | 165 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00474 S00475 S90291 |