論文 - 詳細
| RRC ID | 2904 |
|---|---|
| 著者 | Chen YS, Yanagida F, Hsu JS. |
| タイトル | Isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in Taiwan. |
| ジャーナル | Lett Appl Microbiol |
| Abstract |
AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were isolated from dochi samples and the isolates were divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Phenotypic and biochemical characteristics identified six different bacterial groups (A-F) and showed that the majority of the isolates were homofermentative LAB. Enterococcus faecium was the most abundant of the dochi-isolated LAB. All isolated LAB were able to grow in MRS broth containing 6% NaCl, but only Enterococcus, Pediococcus and Tetragenococcus species could grow in MRS broth containing 10% NaCl. Furthermore, antibacterial activities of isolates were determined, and four isolates showed inhibitory activities against the indicator strain Lactobacillus sakei JCM 1157(T). CONCLUSIONS:These results suggest that Ent. faecium is the main LAB present during the fermentation of dochi. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first report describing the distribution and varieties of LAB that exist in the dochi fermentation process. |
| 巻・号 | 43(2) |
| ページ | 229-35 |
| 公開日 | 2006-8-1 |
| DOI | 10.1111/j.1472-765X.2006.01922.x |
| PII | LAM1922 |
| PMID | 16869910 |
| MeSH | Antibiosis Bacteria / classification* Bacteria / isolation & purification* Enterococcus faecium / classification Enterococcus faecium / isolation & purification Enterococcus faecium / physiology Fermentation Food Microbiology* Lactobacillus / growth & development Microbial Viability Phaseolus / metabolism Phaseolus / microbiology* Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S / genetics Sodium Chloride Streptococcaceae / classification Streptococcaceae / isolation & purification* Streptococcaceae / physiology Taiwan |
| IF | 2.173 |
| 引用数 | 28 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | JCM 1157? |