RRC ID 29059
Author Tsujioka M, Yoshida K, Inouye K.
Title Talin B is required for force transmission in morphogenesis of Dictyostelium.
Journal EMBO J.
Abstract Talin plays a key role in the assembly and stabilisation of focal adhesions, but whether it is directly involved in force transmission during morphogenesis remains to be elucidated. We show that the traction force of Dictyostelium cells mutant for one of its two talin genes talB is considerably smaller than that of wild-type cells, both in isolation and within tissues undergoing morphogenetic movement. The motility of mutant cells in tightly packed tissues in vivo or under strong resistance conditions in vitro was lower than that of wild-type cells, but their motility under low external force conditions was not impaired, indicating inefficient transmission of force in mutant cells. Antibody staining revealed that the talB gene product (talin B) exists as small units subjacent to the cell membrane at adhesion sites without forming large focal adhesion-like assemblies. The total amount of talin B on the cell membrane was larger in prestalk cells, which exert larger force than prespore cells during morphogenesis. We conclude that talin B is involved in force transmission between the cytoskeleton and cell exterior.
Volume 23(11)
Pages 2216-25
Published 2004-6-2
DOI 10.1038/sj.emboj.7600238
PII 7600238
PMID 15141168
PMC PMC419915
MeSH Actins / metabolism Animals Cell Membrane / metabolism Cell Movement Chimera / genetics Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / growth & development* Dictyostelium / metabolism Fluorescent Antibody Technique, Indirect Fluorescent Dyes Focal Adhesions / metabolism Microscopy, Confocal Morphogenesis* Mutation Myosins / metabolism Phalloidine Protein Structure, Tertiary Protozoan Proteins / genetics Protozoan Proteins / metabolism* Rhodamines Talin / chemistry Talin / genetics Talin / metabolism*
IF 10.557
Times Cited 37
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Cellular slime molds S00015 S00121