RRC ID 29087
著者 Sawai S, Guan XJ, Kuspa A, Cox EC.
タイトル High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
ジャーナル Genome Biol
Abstract We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.
巻・号 8(7)
ページ R144
公開日 2007-1-1
DOI 10.1186/gb-2007-8-7-r144
PII gb-2007-8-7-r144
PMID 17659086
PMC PMC2323234
MeSH Animals Dictyostelium / cytology* Dictyostelium / genetics Dictyostelium / growth & development Microscopy, Video / methods* Mutation Phenotype
IF 10.806
引用数 37
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
細胞性粘菌 S00491 S00494 S00495 S00498 S00499 S00500 S00501 S00503 S00504 S00505 S00506 S00507 S00508 S00509 S00510 S00511 S00512 S00513 S00514 S00515 S00516 S00517 S00518 S00519 S00520 S00521 S00522 S00523 S00524 S00525 S00526 S00527 S00530 S00531 S00532 S00533 S00534 S00535 S00536 S90305