Reference - Detail
| RRC ID | 29104 |
|---|---|
| Author | Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL. |
| Title | Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium. |
| Journal | J Biol Chem |
| Abstract |
Phosphorylation of the actin-related protein 2 (Arp2) subunit of the Arp2/3 complex on evolutionarily conserved threonine and tyrosine residues was recently identified and shown to be necessary for nucleating activity of the Arp2/3 complex and membrane protrusion of Drosophila cells. Here we use the Dictyostelium diploid system to replace the essential Arp2 protein with mutants that cannot be phosphorylated at Thr-235/6 and Tyr-200. We found that aggregation of the resulting mutant cells after starvation was substantially slowed with delayed early developmental gene expression and that chemotaxis toward a cAMP gradient was defective with loss of polarity and attenuated F-actin assembly. Chemotaxis toward cAMP was also diminished with reduced cell speed and directionality and shorter pseudopod lifetime when Arp2 phosphorylation mutant cells were allowed to develop longer to a responsive state similar to that of wild-type cells. However, clathrin-mediated endocytosis and chemotaxis under agar to folate in vegetative cells were only subtly affected in Arp2 phosphorylation mutants. Thus, phosphorylation of threonine and tyrosine is important for a subset of the functions of the Arp2/3 complex, in particular an unexpected major role in regulating development. |
| Volume | 288(4) |
| Pages | 2464-74 |
| Published | 2013-1-25 |
| DOI | 10.1074/jbc.M112.435313 |
| PII | S0021-9258(20)46516-0 |
| PMID | 23223240 |
| PMC | PMC3554915 |
| MeSH | Actin Cytoskeleton / metabolism Actin-Related Protein 2 / chemistry* Actins / metabolism Alleles Animals Cell Movement Chemotaxis Cyclic AMP / metabolism* Dictyostelium / metabolism* Endocytosis Models, Biological Mutation Phosphorylation Spectroscopy, Fourier Transform Infrared / methods Tyrosine / chemistry |
| IF | 4.238 |
| Times Cited | 13 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | S90278 |