| RRC ID |
29104
|
| 著者 |
Choi CH, Thomason PA, Zaki M, Insall RH, Barber DL.
|
| タイトル |
Phosphorylation of actin-related protein 2 (Arp2) is required for normal development and cAMP chemotaxis in Dictyostelium.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Phosphorylation of the actin-related protein 2 (Arp2) subunit of the Arp2/3 complex on evolutionarily conserved threonine and tyrosine residues was recently identified and shown to be necessary for nucleating activity of the Arp2/3 complex and membrane protrusion of Drosophila cells. Here we use the Dictyostelium diploid system to replace the essential Arp2 protein with mutants that cannot be phosphorylated at Thr-235/6 and Tyr-200. We found that aggregation of the resulting mutant cells after starvation was substantially slowed with delayed early developmental gene expression and that chemotaxis toward a cAMP gradient was defective with loss of polarity and attenuated F-actin assembly. Chemotaxis toward cAMP was also diminished with reduced cell speed and directionality and shorter pseudopod lifetime when Arp2 phosphorylation mutant cells were allowed to develop longer to a responsive state similar to that of wild-type cells. However, clathrin-mediated endocytosis and chemotaxis under agar to folate in vegetative cells were only subtly affected in Arp2 phosphorylation mutants. Thus, phosphorylation of threonine and tyrosine is important for a subset of the functions of the Arp2/3 complex, in particular an unexpected major role in regulating development.
|
| 巻・号 |
288(4)
|
| ページ |
2464-74
|
| 公開日 |
2013-1-25
|
| DOI |
10.1074/jbc.M112.435313
|
| PII |
S0021-9258(20)46516-0
|
| PMID |
23223240
|
| PMC |
PMC3554915
|
| MeSH |
Actin Cytoskeleton / metabolism
Actin-Related Protein 2 / chemistry*
Actins / metabolism
Alleles
Animals
Cell Movement
Chemotaxis
Cyclic AMP / metabolism*
Dictyostelium / metabolism*
Endocytosis
Models, Biological
Mutation
Phosphorylation
Spectroscopy, Fourier Transform Infrared / methods
Tyrosine / chemistry
|
| IF |
4.238
|
| 引用数 |
13
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
S90278 |
