RRC ID 29122
著者 Beatty A, Morton D, Kemphues K.
タイトル The C. elegans homolog of Drosophila Lethal giant larvae functions redundantly with PAR-2 to maintain polarity in the early embryo.
ジャーナル Development
Abstract Polarity is essential for generating cell diversity. The one-cell C. elegans embryo serves as a model for studying the establishment and maintenance of polarity. In the early embryo, a myosin II-dependent contraction of the cortical meshwork asymmetrically distributes the highly conserved PDZ proteins PAR-3 and PAR-6, as well as an atypical protein kinase C (PKC-3), to the anterior. The RING-finger protein PAR-2 becomes enriched on the posterior cortex and prevents these three proteins from returning to the posterior. In addition to the PAR proteins, other proteins are required for polarity in many metazoans. One example is the conserved Drosophila tumor-suppressor protein Lethal giant larvae (Lgl). In Drosophila and mammals, Lgl contributes to the maintenance of cell polarity and plays a role in asymmetric cell division. We have found that the C. elegans homolog of Lgl, LGL-1, has a role in polarity but is not essential. It localizes asymmetrically to the posterior of the early embryo in a PKC-3-dependent manner, and functions redundantly with PAR-2 to maintain polarity. Furthermore, overexpression of LGL-1 is sufficient to rescue loss of PAR-2 function. LGL-1 negatively regulates the accumulation of myosin (NMY-2) on the posterior cortex, representing a possible mechanism by which LGL-1 might contribute to polarity maintenance.
巻・号 137(23)
ページ 3995-4004
公開日 2010-12-1
DOI 10.1242/dev.056028
PII dev.056028
PMID 21041363
PMC PMC2976283
MeSH Alleles Animals Body Patterning / physiology* Caenorhabditis elegans / cytology* Caenorhabditis elegans / enzymology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Drosophila / metabolism Drosophila Proteins / chemistry* Embryo, Nonmammalian / metabolism* Embryo, Nonmammalian / pathology Epithelial Cells / cytology Epithelial Cells / metabolism Mutation / genetics Myosin Heavy Chains / metabolism Protein Kinase C / metabolism Protein Transport Sequence Homology, Amino Acid Tumor Suppressor Proteins / chemistry*
IF 5.611
引用数 48
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 lgl-1(tm2616) tm2716