RRC ID 29130
Author Kanegae Y, Lee G, Sato Y, Tanaka M, Nakai M, Sakaki T, Sugano S, Saito I.
Title Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
Journal Nucleic Acids Res.
Abstract A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for establishing a powerful on/off switching strategy of gene expression in cultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.
Volume 23(19)
Pages 3816-21
Published 1995-10-11
PII 5w0150
PMID 7479022
PMC PMC307296
MeSH Adenoviridae / genetics* Animals Base Sequence Blotting, Southern Cell Line DNA Nucleotidyltransferases / genetics* Escherichia coli / genetics Gene Expression Regulation* Haplorhini HeLa Cells Humans Integrases* Kidney Molecular Sequence Data Promoter Regions, Genetic Recombinant Proteins / metabolism T-Lymphocytes Transcriptional Activation Transfection Viral Proteins* beta-Galactosidase / genetics beta-Galactosidase / metabolism
IF 11.561
Times Cited 593
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pAxcw (RDB00917) pAxc (RDB00918) pCALNLw (RDB01674) pxCANCre (RDB01675) pxCANLacZ (RDB01676) pULwL (RDB01677) pAxCAwt (RDB01678) pCALwL (RDB01679) pCALNLZ (RDB01680) AxCALacZ (RDB01745) AxCANCre (RDB01748) AxCANLacZ (RDB01749) AxCALNLNZ (RDB01750) pCALNL5 (RDB01862)