| RRC ID |
29130
|
| Author |
Kanegae Y, Lee G, Sato Y, Tanaka M, Nakai M, Sakaki T, Sugano S, Saito I.
|
| Title |
Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the Cre-expressing and the reporter recombinant Ads showed that the Cre-mediated switching of gene expression was detected in nearly 100% of cultured CV1, HeLa and Jurkat cells. These results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for establishing a powerful on/off switching strategy of gene expression in cultured mammalian cells and presumably in transgenic animals. The method is also applicable to construction of recombinant Ad bearing a gene the expression of which is deleterious to propagation of recombinant Ad.
|
| Volume |
23(19)
|
| Pages |
3816-21
|
| Published |
1995-10-11
|
| DOI |
10.1093/nar/23.19.3816
|
| PII |
5w0150
|
| PMID |
7479022
|
| PMC |
PMC307296
|
| MeSH |
Adenoviridae / genetics*
Animals
Base Sequence
Blotting, Southern
Cell Line
DNA Nucleotidyltransferases / genetics*
Escherichia coli / genetics
Gene Expression Regulation*
Haplorhini
HeLa Cells
Humans
Integrases*
Kidney
Molecular Sequence Data
Promoter Regions, Genetic
Recombinant Proteins / metabolism
T-Lymphocytes
Transcriptional Activation
Transfection
Viral Proteins*
beta-Galactosidase / genetics
beta-Galactosidase / metabolism
|
| IF |
11.502
|
| Times Cited |
601
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pAxcw (RDB00917)
pAxc (RDB00918)
pCALNLw (RDB01674)
pxCANCre (RDB01675)
pxCANLacZ (RDB01676)
pULwL (RDB01677)
pAxCAwt (RDB01678)
pCALwL (RDB01679)
pCALNLZ (RDB01680)
AxCALacZ (RDB01745)
AxCANCre (RDB01748)
AxCANLacZ (RDB01749)
AxCALNLNZ (RDB01750)
pCALNL5 (RDB01862) |
