| RRC ID |
29145
|
| 著者 |
Fukamachi K, Matsuoka Y, Kitanaka C, Kuchino Y, Tsuda H.
|
| タイトル |
Rat neuronal leucine-rich repeat protein-3: cloning and regulation of the gene expression.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Rat neuronal leucine-rich repeat protein-3 (rNLRR-3) gene was isolated and cloned from fibrosarcoma cells overexpressing c-Ha-ras. Stable expression of constitutively active forms of Ras (H-Ras(V12) or v-H-Ras) led to a two- to fourfold increase in rNLRR-3 mRNA in rat normal fibroblasts (3Y1). When cells expressing H-Ras(V12) were treated with mitogen activated protein kinase (MAPK) kinase inhibitors (U0126, PD98059), suppression of rNLRR-3 mRNA correlated well with a reduction in MAPK activity. Epidermal growth factor (EGF) led to elevation of rNLRR-3 gene expression about 4 h after stimulation of normal fibroblasts. U0126 completely suppressed the induction by EGF of rNLRR-3 mRNA with abrogation of MAPK phosphorylation. U0126 inhibited the basal transcription of rNLRR-3. LY294002, a PI3 kinase inhibitor, showed a lesser effect on expression of the gene. These results indicate that rNLRR-3 gene expression is regulated mainly through the Ras-MAPK signaling pathway in fibroblasts.
|
| 巻・号 |
287(1)
|
| ページ |
257-63
|
| 公開日 |
2001-9-14
|
| DOI |
10.1006/bbrc.2001.5579
|
| PII |
S0006-291X(01)95579-2
|
| PMID |
11549284
|
| MeSH |
Amino Acid Sequence
Animals
Cell Line, Transformed
Cells, Cultured
Cloning, Molecular
DNA, Complementary / analysis
Gene Expression Regulation*
Humans
Mitogen-Activated Protein Kinases / metabolism
Molecular Sequence Data
Nerve Tissue Proteins / genetics*
Rats
Sequence Homology, Amino Acid
Up-Regulation
ras Proteins / metabolism
|
| IF |
2.985
|
| 引用数 |
18
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pUSE-NLRR3 (RDB03437) |
