RRC ID 29145
Author Fukamachi K, Matsuoka Y, Kitanaka C, Kuchino Y, Tsuda H.
Title Rat neuronal leucine-rich repeat protein-3: cloning and regulation of the gene expression.
Journal Biochem Biophys Res Commun
Abstract Rat neuronal leucine-rich repeat protein-3 (rNLRR-3) gene was isolated and cloned from fibrosarcoma cells overexpressing c-Ha-ras. Stable expression of constitutively active forms of Ras (H-Ras(V12) or v-H-Ras) led to a two- to fourfold increase in rNLRR-3 mRNA in rat normal fibroblasts (3Y1). When cells expressing H-Ras(V12) were treated with mitogen activated protein kinase (MAPK) kinase inhibitors (U0126, PD98059), suppression of rNLRR-3 mRNA correlated well with a reduction in MAPK activity. Epidermal growth factor (EGF) led to elevation of rNLRR-3 gene expression about 4 h after stimulation of normal fibroblasts. U0126 completely suppressed the induction by EGF of rNLRR-3 mRNA with abrogation of MAPK phosphorylation. U0126 inhibited the basal transcription of rNLRR-3. LY294002, a PI3 kinase inhibitor, showed a lesser effect on expression of the gene. These results indicate that rNLRR-3 gene expression is regulated mainly through the Ras-MAPK signaling pathway in fibroblasts.
Volume 287(1)
Pages 257-63
Published 2001-9-14
DOI 10.1006/bbrc.2001.5579
PII S0006-291X(01)95579-2
PMID 11549284
MeSH Amino Acid Sequence Animals Cell Line, Transformed Cells, Cultured Cloning, Molecular DNA, Complementary / analysis Gene Expression Regulation* Humans Mitogen-Activated Protein Kinases / metabolism Molecular Sequence Data Nerve Tissue Proteins / genetics* Rats Sequence Homology, Amino Acid Up-Regulation ras Proteins / metabolism
IF 2.985
Times Cited 18
DNA material pUSE-NLRR3 (RDB03437)