論文 - 詳細
| RRC ID | 29145 |
|---|---|
| 著者 | Fukamachi K, Matsuoka Y, Kitanaka C, Kuchino Y, Tsuda H. |
| タイトル | Rat neuronal leucine-rich repeat protein-3: cloning and regulation of the gene expression. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Rat neuronal leucine-rich repeat protein-3 (rNLRR-3) gene was isolated and cloned from fibrosarcoma cells overexpressing c-Ha-ras. Stable expression of constitutively active forms of Ras (H-Ras(V12) or v-H-Ras) led to a two- to fourfold increase in rNLRR-3 mRNA in rat normal fibroblasts (3Y1). When cells expressing H-Ras(V12) were treated with mitogen activated protein kinase (MAPK) kinase inhibitors (U0126, PD98059), suppression of rNLRR-3 mRNA correlated well with a reduction in MAPK activity. Epidermal growth factor (EGF) led to elevation of rNLRR-3 gene expression about 4 h after stimulation of normal fibroblasts. U0126 completely suppressed the induction by EGF of rNLRR-3 mRNA with abrogation of MAPK phosphorylation. U0126 inhibited the basal transcription of rNLRR-3. LY294002, a PI3 kinase inhibitor, showed a lesser effect on expression of the gene. These results indicate that rNLRR-3 gene expression is regulated mainly through the Ras-MAPK signaling pathway in fibroblasts. |
| 巻・号 | 287(1) |
| ページ | 257-63 |
| 公開日 | 2001-9-14 |
| DOI | 10.1006/bbrc.2001.5579 |
| PII | S0006-291X(01)95579-2 |
| PMID | 11549284 |
| MeSH | Amino Acid Sequence Animals Cell Line, Transformed Cells, Cultured Cloning, Molecular DNA, Complementary / analysis Gene Expression Regulation* Humans Mitogen-Activated Protein Kinases / metabolism Molecular Sequence Data Nerve Tissue Proteins / genetics* Rats Sequence Homology, Amino Acid Up-Regulation ras Proteins / metabolism |
| IF | 2.985 |
| 引用数 | 18 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pUSE-NLRR3 (RDB03437) |