論文 - 詳細
| RRC ID | 29146 |
|---|---|
| 著者 | Fukamachi K, Matsuoka Y, Ohno H, Hamaguchi T, Tsuda H. |
| タイトル | Neuronal leucine-rich repeat protein-3 amplifies MAPK activation by epidermal growth factor through a carboxyl-terminal region containing endocytosis motifs. |
| ジャーナル | J Biol Chem |
| Abstract |
Neuronal leucine-rich repeat protein-3 (NLRR-3) belongs to the LRR superfamily. Expression of rat NLRR-3 gene isolated from c-Ha-ras transgenic rat tumor is regulated mainly through the Ras-MAPK signaling pathway. NLRR-3 was found to enhance phosphorylation of MAPK when COS-7 cells were transfected with NLRR-3 and stimulated with a low concentration (0.01 ng/ml) of epidermal growth factor (EGF), but the amplification of MAPK phosphorylation by NLRR-3 was no longer observed when the carboxyl-terminal 30 amino acid stretch containing clathrin-mediated endocytosis motifs was deleted. A green fluorescent protein-tagged NLRR-3 localized at the plasma membrane was efficiently internalized in COS-7 cells, but internalization of a carboxyl-terminal-deleted version (NLRRDeltaC) was less efficient. The presence of clathrin-adaptor protein complexes containing NLRR-3 in brain lysate was confirmed by immunoprecipitation and glutathione S-transferase pull-down experiments, and affinity column chromatography revealed that the carboxyl-terminal region of NLRR-3 interacts with beta-adaptin. We propose that NLRR-3 potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles. |
| 巻・号 | 277(46) |
| ページ | 43549-52 |
| 公開日 | 2002-11-15 |
| DOI | 10.1074/jbc.C200502200 |
| PII | S0021-9258(19)71686-X |
| PMID | 12297494 |
| MeSH | 3T3 Cells Adaptor Protein Complex beta Subunits / metabolism Amino Acid Motifs Amino Acid Sequence Animals Blotting, Western Brain / metabolism COS Cells Endocytosis Enzyme Activation Epidermal Growth Factor / metabolism* Escherichia coli / metabolism Glutathione Transferase / metabolism Leucine / chemistry* MAP Kinase Signaling System* Membrane Glycoproteins Mice Microscopy, Fluorescence Models, Biological Molecular Sequence Data Nerve Tissue Proteins / metabolism Nerve Tissue Proteins / physiology* Phosphorylation Plasmids / metabolism Precipitin Tests Protein Binding Protein Structure, Tertiary Rats Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Time Factors Transfection |
| IF | 4.238 |
| 引用数 | 34 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pUSE-NLRR3 (RDB03437) |