RRC ID 29159
Author Nishii A, Takemura M, Fujita H, Shikata M, Yokota A, Kohchi T.
Title Characterization of a novel gene encoding a putative single zinc-finger protein, ZIM, expressed during the reproductive phase in Arabidopsis thaliana.
Journal Biosci. Biotechnol. Biochem.
Abstract By differential screening of an arrayed normalized cDNA library from the inflorescence apex in Arabidopsis, a cDNA clone having a deduced amino acid sequence with a motif for a zinc finger was isolated as one of the genes expressed specifically in the reproductive phase. The deduced protein has a modular structure with a putative single C2-C2 zinc-finger motif distantly related to a GATA-1-type finger, a basic region with a sequence resembling a nuclear localization signal, and an acidic region. The gene seemed to have been formed by the exon-shuffling during its molecular evolution, since individual domains are encoded by discrete exons. RNA gel blot analysis showed its expression in shoot apex and flowers in the reproductive phase. The gene was named ZIM for Zinc-finger protein expressed in Inflorescence Meristem. The nuclear localization of ZIM was detected using GFP as a reporter. These results suggest that ZIM is a putative transcription factor involved in inflorescence and flower development.
Volume 64(7)
Pages 1402-9
Published 2000-7
DOI 10.1271/bbb.64.1402
PMID 10945256
MeSH Amino Acid Sequence Arabidopsis / genetics Arabidopsis Proteins* Base Sequence Cell Nucleus / metabolism Cloning, Molecular DNA, Complementary DNA, Plant Gene Expression Gene Library Genes, Plant* Genome, Plant Molecular Sequence Data Plant Proteins / genetics* Sequence Homology, Amino Acid Transcription Factors / genetics* Zinc Fingers*
IF 1.255
Times Cited 42
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material ZIM (RDB02996)