論文 - 詳細
| RRC ID | 29166 |
|---|---|
| 著者 | Yamanushi T, Hirata A, Oka T, Nakano A. |
| タイトル | Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum. |
| ジャーナル | J Biochem |
| Abstract |
SAR1 encodes a low molecular weight GTPase that is essential in the early process of vesicular transport in the secretory pathway. By random and site-directed mutagenesis of the SAR1 gene, we have obtained three temperature-sensitive mutants, N132I, E112K, and D32G. They all show a defect in transport from the endoplasmic reticulum to the Golgi apparatus, and accumulate endoplasmic reticulum membranes at the restrictive temperature. This is consistent with our previous observations in vivo on a galactose-shutoff mutant as well as the in vitro results, and provides powerful tools for further genetic analyses. |
| 巻・号 | 120(2) |
| ページ | 452-8 |
| 公開日 | 1996-8-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a021432 |
| PMID | 8889833 |
| MeSH | Alleles Amino Acid Sequence Biological Transport, Active Endoplasmic Reticulum / metabolism Escherichia coli / genetics Fungal Proteins / metabolism GTP-Binding Proteins / genetics* GTP-Binding Proteins / metabolism* Genes, Fungal Golgi Apparatus / metabolism Microscopy, Electron Molecular Sequence Data Monomeric GTP-Binding Proteins* Mutagenesis Mutation Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae / ultrastructure Saccharomyces cerevisiae Proteins* Temperature Vesicular Transport Proteins |
| IF | 2.476 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | pTY3 RDB08914) pTY5 (RDB08915) pMYT3-7 (rDB08917) |