Reference - Detail
|Author||Yamanushi T, Hirata A, Oka T, Nakano A.|
|Title||Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum.|
SAR1 encodes a low molecular weight GTPase that is essential in the early process of vesicular transport in the secretory pathway. By random and site-directed mutagenesis of the SAR1 gene, we have obtained three temperature-sensitive mutants, N132I, E112K, and D32G. They all show a defect in transport from the endoplasmic reticulum to the Golgi apparatus, and accumulate endoplasmic reticulum membranes at the restrictive temperature. This is consistent with our previous observations in vivo on a galactose-shutoff mutant as well as the in vitro results, and provides powerful tools for further genetic analyses.
|MeSH||Alleles Amino Acid Sequence Biological Transport, Active Endoplasmic Reticulum / metabolism Escherichia coli / genetics Fungal Proteins / metabolism GTP-Binding Proteins / genetics* GTP-Binding Proteins / metabolism* Genes, Fungal Golgi Apparatus / metabolism Microscopy, Electron Molecular Sequence Data Monomeric GTP-Binding Proteins* Mutagenesis Mutation Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae / ultrastructure Saccharomyces cerevisiae Proteins* Temperature Vesicular Transport Proteins|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY|
|DNA material||pTY3 RDB08914) pTY5 (RDB08915) pMYT3-7 (rDB08917)|