RRC ID 29186
Author Horikawa Y, Matsumoto H, Yamaguchi F, Ishida S, Fujiwara S.
Title Transcriptional regulation in the early ectodermal lineage of ascidian embryos.
Journal Dev Growth Differ
Abstract In ascidian embryos, ectodermal tissues derive from blastomeres in the animal hemisphere. The animal hemisphere-specific gene expression is observed as early as the 16-cell stage. Here, we characterized animal hemisphere-specific enhancers of three genes, Ci-ephrin-Ad, Ci-TGFβ-NA1 and Ci-Fz4. Deletion analyses identified minimal essential elements. Although these elements contained multiple GATA sequences, electrophoretic mobility shift assays revealed that only some of them were strong binding sites for the transcription factor Ci-GATAa. On the other hand, the motif-searching software MEME identified an octamer, GA (T/G) AAGGG, shared by these enhancers. In Ci-ephrin-Ad and Ci-TGFβ-NA1, the octamer was GATAAGGG, which strongly bound Ci-GATAa. The 397-bp upstream region of Ci-ephrin-Ad contained two strong Ci-GATAa-binding sites, one of which was the octamer motif. Mutation in the octamer motif, but not the other Ci-GATAa-binding site, severely affected the enhancer activity. The 204-bp upstream region of Ci-TGFβ-NA1 contained four strong Ci-GATAa-binding sites, including the octamer motif. Mutation only in the octamer motif, leaving the other three Ci-GATAa-binding sites intact, abolished the enhancer activity. These results suggest a crucial role for the octamer motif.
Volume 55(9)
Pages 776-85
Published 2013-12-1
DOI 10.1111/dgd.12100
PMID 24192291
MeSH Animals Binding Sites / genetics Blastomeres / metabolism Cell Lineage / physiology* Ciona intestinalis / embryology* DNA Primers / genetics Ectoderm / embryology* Ectoderm / metabolism Electrophoretic Mobility Shift Assay Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology*
IF 1.723
Times Cited 4
DNA material Ephrin1648Z (RDB13055) Tgfna2940Z (RDB13056) Fz1964Z (RDB13057)
C.intestinalis / (O.japonicus) Wild C. int