RRC ID 29188
著者 Kanda M, Ikeda T, Fujiwara S.
タイトル Identification of a retinoic acid-responsive neural enhancer in the Ciona intestinalis Hox1 gene.
ジャーナル Dev. Growth Differ.
Abstract The Hox1 gene in the urochordate ascidian Ciona intestinalis (Ci-Hox1) is expressed in the nerve cord and epidermis. We identified a nerve cord enhancer in the second intron of Ci-Hox1, and demonstrated that retinoic acid (RA) plays a major role in activating this enhancer. The enhancer contained a putative retinoic acid-response element (RARE). Mutation of the RARE in the Ci-Hox1 nerve cord enhancer only partially abolished the enhancer activity. Genes encoding RA synthase and the RA receptor were knocked down using specific antisense morpholino oligos (MOs), and injection of embryos with these MOs resulted in the complete disappearance of epidermal expression of Ci-Hox1 and reduction of neural expression. However, nerve cord expression was not completely repressed. These results suggest that the nerve cord enhancer is activated by two partially redundant pathways; one RA-dependent and one RA-independent.
巻・号 55(2)
ページ 260-9
公開日 2013-1-11
DOI 10.1111/dgd.12033
PMID 23302037
MeSH Animals Central Nervous System / embryology Central Nervous System / metabolism Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Enhancer Elements, Genetic / genetics* Gene Expression Regulation, Developmental / drug effects* Gene Knockdown Techniques Homeodomain Proteins / genetics* In Situ Hybridization Mutation Receptors, Retinoic Acid / genetics Receptors, Retinoic Acid / metabolism Retinal Dehydrogenase / genetics Retinal Dehydrogenase / metabolism Tretinoin / pharmacology*
IF 2.145
引用数 4
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int