RRC ID 29198
著者 Choi YL, Nishida T, Kawamukai M, Utsumi R, Sakai H, Komano T.
タイトル Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108.
ジャーナル J Bacteriol
Abstract An nlp (Ner-like protein) gene was isolated from Escherichia coli. The nucleotide sequence of a 1,342-base-pair chromosomal DNA fragment containing the nlp gene was analyzed. It contained two open reading frames; one encoded 91 amino acid residues with an Mr of 10,361, and the other (ORFX) encoded 131 amino acid residues of the carboxyl-terminal region of a truncated polypeptide. The amino acid sequence deduced from the DNA sequence of nlp was highly homologous (62 to 63%) to the Ner proteins of bacteriophages Mu and D108. The amino-terminal region of Nlp deduced from the complete open reading frame contained a presumed DNA-binding region. The nlp gene was located at 69.3 min on the E. coli genetic map.
巻・号 171(9)
ページ 5222-5
公開日 1989-9-1
DOI 10.1128/jb.171.9.5222-5225.1989
PMID 2670911
PMC PMC210348
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular Coliphages / genetics* DNA-Binding Proteins / genetics* Escherichia coli / genetics* Genes* Genes, Bacterial* Genes, Viral* Molecular Sequence Data Repressor Proteins / genetics* Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors / genetics* Viral Proteins / genetics* Viral Regulatory and Accessory Proteins
IF 3.006
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
遺伝子材料 pKA3 (RDB01546) pKA7 (RDB01547)