RRC ID 29203
Author Nakashima N, Tanaka K, Sturm S, Okayama H.
Title Fission yeast Rep2 is a putative transcriptional activator subunit for the cell cycle 'start' function of Res2-Cdc10.
Journal EMBO J
Abstract In the yeast cell cycle 'start' requires sets of the Cdc10/ SWI family of transcriptional factors which activate the MCB cis elements contained in genes essential for S phase progression. Fission yeast possess two such overlapping systems, Res1-Cdc10 and Res2-Cdc10, both of which act to start the mitotic and meiotic cycles. We have recently isolated rep2+ as a multicopy suppressor of a temperature-sensitive cdc10 mutant which encodes a zinc finger protein. Here we show that the Rep2 zinc finger protein is an essential component of the active Res2-Cdc10 transcriptional regulator complex and likely to play a role in the control of cell cycle 'start'. Our data suggest that Rep2 is a transcriptional activator subunit which interacts with the MCB binding subunit complex formed by Res2 and Cdc10.
Volume 14(19)
Pages 4794-802
Published 1995-10-2
PMID 7588609
PMC PMC394577
MeSH Amino Acid Sequence Base Sequence Cell Cycle Proteins / metabolism* Cell Cycle Proteins / physiology Cloning, Molecular DNA / metabolism DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Fungal Proteins / genetics Fungal Proteins / metabolism* Fungal Proteins / physiology G1 Phase / genetics* Genes, Fungal / genetics Genes, Regulator / genetics Meiosis / genetics Mitosis / genetics Models, Genetic Molecular Sequence Data Nitrogen / physiology Schizosaccharomyces / genetics Schizosaccharomyces / growth & development Schizosaccharomyces pombe Proteins* Sequence Analysis, DNA Sequence Homology, Amino Acid Suppression, Genetic Trans-Activators / chemistry Trans-Activators / genetics Trans-Activators / metabolism Trans-Activators / physiology* Transcription Factors Zinc Fingers / genetics
IF 9.889
Times Cited 55
DNA material rep2^+ (RDB01609)