RRC ID 29299
Author Kumoi K, Satoh T, Murata K, Hiromoto T, Mizushima T, Kamiya Y, Noda M, Uchiyama S, Yagi H, Kato K.
Title An archaeal homolog of proteasome assembly factor functions as a proteasome activator.
Journal PLoS ONE
Abstract Assembly of the eukaryotic 20S proteasome is an ordered process involving several proteins operating as proteasome assembly factors including PAC1-PAC2 but archaeal 20S proteasome subunits can spontaneously assemble into an active cylindrical architecture. Recent bioinformatic analysis identified archaeal PAC1-PAC2 homologs PbaA and PbaB. However, it remains unclear whether such assembly factor-like proteins play an indispensable role in orchestration of proteasome subunits in archaea. We revealed that PbaB forms a homotetramer and exerts a dual function as an ATP-independent proteasome activator and a molecular chaperone through its tentacle-like C-terminal segments. Our findings provide insights into molecular evolution relationships between proteasome activators and assembly factors.
Volume 8(3)
Pages e60294
Published 2013
DOI 10.1371/journal.pone.0060294
PII PONE-D-12-23454
PMID 23555947
PMC PMC3605417
MeSH Archaea / metabolism* Archaeal Proteins / metabolism* Evolution, Molecular Proteasome Endopeptidase Complex / metabolism* Protein Binding
IF 2.766
Times Cited 8
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material Genomic DNA of Pyrococcus furiosus JCM 8422T (JGD07704)
General Microbes JCM 8422