RRC ID 29402
著者 Nimaichand S, Zhang YG, Cheng J, Li L, Zhang DF, Zhou EM, Dong L, Ningthoujam DS, Li WJ.
タイトル Micromonospora kangleipakensis sp. nov., isolated from a sample of limestone quarry.
ジャーナル Int J Syst Evol Microbiol
Abstract Strain MBRL 34(T), isolated from a sample of limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain showed the highest 16S rRNA gene sequence similarity with Micromonospora echinaurantica DSM 43904(T) (98.4 %), but formed a monophyletic clade with Micromonospora coerulea DSM 43143(T) (98.3 %) in the neighbour-joining tree. DNA-DNA hybridization experiments gave a DNA-DNA relatedness value of 53.1 % between MBRL 34(T) and M. coerulea DSM 43143(T). Strain MBRL 34(T) contained meso-diaminopimelic acid, galactose and glucose in the whole-cell hydrolysates along with small amounts of mannose, xylose, rhamnose and ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, along with an unknown lipid. MK-10(H6), MK-10(H2) MK-11(H4) and MK-10(H4) were the predominant menaquinones detected. The major fatty acids were iso-C16 : 0 and iso-C15 : 0. The G+C content of the genomic DNA was 73.5 %. Based on the taxonomic characteristics from a polyphasic study, strain MBRL 34(T) merits recognition as a representative of a novel species of the genus Micromonospora for which the name Micromonospora kangleipakensis sp. nov. is proposed; the type strain is MBRL 34(T) ( = DSM 45612(T) = JCM 17696(T)).
巻・号 63(Pt 12)
ページ 4546-4551
公開日 2013-12-1
DOI 10.1099/ijs.0.052746-0
PMID 23907218
MeSH Bacterial Typing Techniques Base Composition Calcium Carbonate* DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry India Micromonospora / classification* Micromonospora / genetics Micromonospora / isolation & purification Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Vitamin K 2 / chemistry
IF 2.415
引用数 8
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 17696