RRC ID 29442
Author Fujii K, Satomi M, Fukui Y, Matsunobu S, Morifuku Y, Enokida Y.
Title Streptomyces abietis sp. nov., a cellulolytic bacterium isolated from soil of a pine forest.
Journal Int J Syst Evol Microbiol
Abstract Cellulolytic bacteria A191(T), A192 and A193 isolated from the soil of Sakhalin fir forest in Hokkaido, Japan were studied phenotypically, genotypically and phylogenetically. Analysis of their 16S rRNA gene and gyrB sequences and DNA base composition suggested that these isolates were conspecific and members of the genus Streptomyces. However, levels of 16S rRNA gene and gyrB sequence similarity between the isolates and the type strains of their closest relatives in the genus Streptomyces were no higher than 97.9 and 95.0 %, respectively, implying that these isolates were distinctive. Moreover, the results of DNA-DNA hybridization experiments and physiological characterization clearly differentiated these isolates from their closest neighbours. It is therefore concluded that these isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces abietis is proposed. The type strain is A191(T) ( = NBRC 109094(T) = DSM 42080(T)).
Volume 63(Pt 12)
Pages 4754-4759
Published 2013-12-1
DOI 10.1099/ijs.0.053025-0
PMID 23990653
MeSH Abies / microbiology* Bacterial Typing Techniques Base Composition DNA Gyrase / genetics DNA, Bacterial / genetics Fatty Acids / chemistry Japan Molecular Sequence Data Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Streptomyces / classification* Streptomyces / genetics Streptomyces / isolation & purification Trees / microbiology
IF 2.415
Times Cited 5
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 14344 JCM 16122