| RRC ID |
29679
|
| 著者 |
Kayukawa T, Murata M, Kobayashi I, Muramatsu D, Okada C, Uchino K, Sezutsu H, Kiuchi M, Tamura T, Hiruma K, Ishikawa Y, Shinoda T.
|
| タイトル |
Hormonal regulation and developmental role of Krüppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori.
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| ジャーナル |
Dev Biol
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| Abstract |
Juvenile hormone (JH) has an ability to repress the precocious metamorphosis of insects during their larval development. Krüppel homolog 1 (Kr-h1) is an early JH-inducible gene that mediates this action of JH; however, the fine hormonal regulation of Kr-h1 and the molecular mechanism underlying its antimetamorphic effect are little understood. In this study, we attempted to elucidate the hormonal regulation and developmental role of Kr-h1. We found that the expression of Kr-h1 in the epidermis of penultimate-instar larvae of the silkworm Bombyx mori was induced by JH secreted by the corpora allata (CA), whereas the CA were not involved in the transient induction of Kr-h1 at the prepupal stage. Tissue culture experiments suggested that the transient peak of Kr-h1 at the prepupal stage is likely to be induced cooperatively by JH derived from gland(s) other than the CA and the prepupal surge of ecdysteroid, although involvement of unknown factor(s) could not be ruled out. To elucidate the developmental role of Kr-h1, we generated transgenic silkworms overexpressing Kr-h1. The transgenic silkworms grew normally until the spinning stage, but their development was arrested at the prepupal stage. The transgenic silkworms from which the CA were removed in the penultimate instar did not undergo precocious pupation or larval-larval molt but fell into prepupal arrest. This result demonstrated that Kr-h1 is indeed involved in the repression of metamorphosis but that Kr-h1 alone is incapable of implementing normal larval molt. Moreover, the expression profiles and hormonal responses of early ecdysone-inducible genes (E74, E75, and Broad) in transgenic silkworms suggested that Kr-h1 is not involved in the JH-dependent modulation of these genes, which is associated with the control of metamorphosis.
|
| 巻・号 |
388(1)
|
| ページ |
48-56
|
| 公開日 |
2014-4-1
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| DOI |
10.1016/j.ydbio.2014.01.022
|
| PII |
S0012-1606(14)00050-5
|
| PMID |
24508345
|
| MeSH |
Animals
Animals, Genetically Modified
Bombyx / embryology*
Bombyx / genetics
Bombyx / metabolism
Ecdysone / chemistry
Ecdysteroids / chemistry
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Insect Proteins / chemistry
Insect Proteins / genetics
Insect Proteins / metabolism*
Kruppel-Like Transcription Factors / genetics
Kruppel-Like Transcription Factors / metabolism*
Larva / genetics
Larva / metabolism
Male
Metamorphosis, Biological*
Open Reading Frames
Signal Transduction
|
| IF |
2.896
|
| 引用数 |
38
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| カイコ |
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