| RRC ID |
29696
|
| 著者 |
Xu J, Mon H, Kusakabe T, Li Z, Zhu L, Iiyama K, Masuda A, Mitsudome T, Lee JM.
|
| タイトル |
Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAi-mediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.
|
| 巻・号 |
97(24)
|
| ページ |
10435-44
|
| 公開日 |
2013-12-1
|
| DOI |
10.1007/s00253-013-5279-x
|
| PMID |
24081323
|
| MeSH |
Animals
Baculoviridae / genetics
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Culture Techniques
Glycoproteins / genetics
Glycoproteins / metabolism*
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Molecular Biology / methods*
Molecular Sequence Data
Nucleopolyhedroviruses / genetics
RNA Interference*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Analysis, DNA
Sf9 Cells
Spodoptera
|
| IF |
3.53
|
| 引用数 |
11
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| カイコ |
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