論文 - 詳細
| RRC ID | 29696 |
|---|---|
| 著者 | Xu J, Mon H, Kusakabe T, Li Z, Zhu L, Iiyama K, Masuda A, Mitsudome T, Lee JM. |
| タイトル | Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAi-mediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS. |
| 巻・号 | 97(24) |
| ページ | 10435-44 |
| 公開日 | 2013-12-1 |
| DOI | 10.1007/s00253-013-5279-x |
| PMID | 24081323 |
| MeSH | Animals Baculoviridae / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Culture Techniques Glycoproteins / genetics Glycoproteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Biology / methods* Molecular Sequence Data Nucleopolyhedroviruses / genetics RNA Interference* Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Analysis, DNA Sf9 Cells Spodoptera |
| IF | 3.53 |
| 引用数 | 11 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
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