論文 - 詳細
| RRC ID | 29744 |
|---|---|
| 著者 | Hafner EW, Tabor CW, Tabor H. |
| タイトル | Isolation of a metK mutant with a temperature-sensitive S-adenosylmethionine synthetase. |
| ジャーナル | J Bacteriol |
| Abstract |
An Escherichia coli metK mutant, designated metK110, was isolated among spontaneous ethionine-resistant organisms selected at 42 degrees C. The S-adenosylmethionine synthetase activity of this mutant was present at lower levels than in the corresponding wild-type strain and was more labile than the wild-type enzyme when heated or dialyzed. A mixture of mutant and wild-type enzyme preparations had an activity equal to the sum of the component activities. These facts strongly suggest that the mutated gene in this strain is the structural gene for this enzyme. Genetic mapping experiments placed the metK110 mutation near or at the site of other known metK mutants (i.e., 63 min), confirming its designation as a metK mutant. A revised gene order has been established for this region, i.e., metC glc speC metK speB serA. |
| 巻・号 | 132(3) |
| ページ | 832-40 |
| 公開日 | 1977-12-1 |
| DOI | 10.1128/jb.132.3.832-840.1977 |
| PMID | 336609 |
| PMC | PMC235585 |
| MeSH | Chromosome Mapping Chromosomes, Bacterial* Escherichia coli / genetics* Escherichia coli / ultrastructure Genes* Methionine Adenosyltransferase / biosynthesis* Mutation Temperature Transferases / biosynthesis* |
| IF | 3.006 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JE6662 JE6667 |