論文 - 詳細
| RRC ID | 29767 |
|---|---|
| 著者 | Novel M, Novel G. |
| タイトル | Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression. |
| ジャーナル | J Bacteriol |
| Abstract |
All methyl-beta-D-galacturonide-positive mutants isolated from Escherichia coli K-12 carry constitutive mutations for beta-glucuronidase (UID) synthesis. Most of these mutants are specific for UID synthesis and are distributed in three classes according to the derepression level of UID. Each specific mutant carries a mutation(s) near uidA, the structural gene for UID, at min 30.5 of the E. coli K-12 linkage map. The expression of UID synthesis in F-merodiploid strains carrying these mutations permits discrimination between dominant and recessive constitutivity over the wild-type allele. The first kind of mutation (dominant) should affect the operator site uidO of the structural gene uidA; the second type of mutation (recessive) should affect a regulatory gene, uidR, operating through a negative control. The isolation of mutants bearing at this locus superrepressed mutations, which can revert to produce a constitutive phenotype, confirms the occurrence of such a regulatory gene. The partially derepressed uidR mutants of the first class are normally inducible and remain constitutive at low temperature; their UID has the same thermal sensitivity as in the wild-type strains. The occurrence of similar regulatory gene mutants has been recently described in the lactose system (Shineberg, 1974). |
| 巻・号 | 127(1) |
| ページ | 406-17 |
| 公開日 | 1976-7-1 |
| DOI | 10.1128/jb.127.1.406-417.1976 |
| PMID | 776933 |
| PMC | PMC233074 |
| MeSH | Cell-Free System Chromosome Mapping Enzyme Repression Escherichia coli / enzymology* Escherichia coli / metabolism Ethyl Methanesulfonate Genes* Genes, Regulator Glucuronidase / biosynthesis* Glucuronidase / metabolism Hexuronic Acids / metabolism Methylnitronitrosoguanidine Mutagens Mutation* Nitrites Temperature Ultraviolet Rays |
| IF | 3.006 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8629 |