RRC ID 29775
Author Spencer ME, Lebeter VM, Guest JR.
Title Location of the Aspartase Gene (aspA) on the linkage map of Escherichia coli K12.
Journal J Gen Microbiol
Abstract The aspartase (L-asparatate ammonia-lyase, EC. 4.3.I.I) structural gene, aspA, was mapped by two-factor and three-factor transductional crosses using phage PI. Cotransduction frequencies between aspA and other markers were: ampA (69%); frdA (48 TO 67%) mel (35%); purA (17%); fdp (I-6%). The sequence of markers within the corresponding segment (91 to 95 min) of the Escherichia coli linkage map was shown to be mel-aspA-ampA-frdA-purA-fdp.
Volume 97(1)
Pages 73-82
Published 1976-11-1
DOI 10.1099/00221287-97-1-73
PMID 792395
MeSH Ammonia-Lyases / biosynthesis* Aspartate Ammonia-Lyase / biosynthesis* Chromosome Mapping Crosses, Genetic Escherichia coli / enzymology* Escherichia coli / metabolism Genes* Genetic Linkage* Glutamates / metabolism Mutation Transduction, Genetic
Resource
Prokaryotes E. coli ME8770