Reference - Detail
| RRC ID | 29776 |
|---|---|
| Author | Bloom FR, McFall E, Young MC, Carothers AM. |
| Title | Positive control in the D-serine deaminase system of Escherichia coli K-12. |
| Journal | J Bacteriol |
| Abstract |
Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator negative) mutations. The mutations lie in the dsdC region, are cis and trans recessive to dsdC+, and give rise to revertants of novel regulatory phenotype. The second class consists of Dsdase-negative lysogens in which the phenotype is the result of the integration of lambdac1857 Sam7 into the dsdC region. Lambda lysates derived from two of the Dsdase-negative lysogens can transduce the structural gene for Dsdase (dsdA) but not the dsdC region. The dsdC+ gene product had no repressor effect on constitutive synthesis in a strain containing a dsdO (initiator constitutive) and a dsdC- mutation. These and other findings indicate that control of Dsdase synthesis is strictly positive. The partial trans effect of the dsdC+ gene product on constitutive synthesis in dsdCc (regulator constitutive) strains can thus be explained by "subunit mixing" between active dsdCc subunits and dsdC+ subunits which are inactive in the absence of the inducer, D-serine. The order of genes in the dsd region is supN-dsdC-dsdP-dsdA-aroC. |
| Volume | 121(3) |
| Pages | 1092-101 |
| Published | 1975-3-1 |
| DOI | 10.1128/jb.121.3.1092-1101.1975 |
| PMID | 1090589 |
| PMC | PMC246040 |
| MeSH | Chromosome Mapping Coliphages / growth & development Enzyme Induction Enzyme Repression Escherichia coli / enzymology* Escherichia coli / isolation & purification Escherichia coli / metabolism Genes Genes, Recessive Genes, Regulator Genetic Linkage Glucose / metabolism Glycerol / metabolism L-Serine Dehydratase / biosynthesis* Lysogeny Mutagens Mutation* Nitrosoguanidines Phenotype Serine / metabolism Stereoisomerism Temperature Transduction, Genetic Virus Replication |
| IF | 3.006 |
| Resource | |
| Prokaryotes E. coli | ME5386 ME5385 |